✂️ Best AI tools for CRISPR & gene editing (2026)

CRISPR guide and pegRNA design, editing-outcome analysis and AI-designed gene editors.

6 tools · 3 open source · each entry links to its website, code and paper.

Dated by release date, else first public paper, preprint or release date; undated tools listed after, A–Z.

OpenCRISPR-1

AI-designed Cas9-like gene editor from Profluent's language models, released openly.

CRISPR & gene editingOpen source#generative#gene-editor

PRIDICT

Attention-based model predicting prime editing efficiency to design pegRNAs.

CRISPR & gene editingOpen source#prime-editing#pegrna

Rule Set 3 (rs3)

Broad GPP model for SpCas9 sgRNA on-target activity, used in CRISPick.

CRISPR & gene editingOpen source#guide-design#on-target

CHOPCHOP

Web tool for designing CRISPR-Cas9, Cas12 and TALEN targets with efficiency predictions.

CRISPR & gene editingFree (research)#guide-design#web

CRISPResso2

Analysis of genome-editing outcomes from amplicon sequencing, including base and prime editing.

CRISPR & gene editingOpen source (non-commercial)#editing-outcomes#amplicon

CRISPOR

Web tool for CRISPR guide selection with on-target efficiency and off-target scoring for 500+ genomes.

CRISPR & gene editingOpen source (non-commercial)#guide-design#off-target